Composite

Part:BBa_K781003:Design

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)


[R0010][B0034] - Cohesin II-FliC Insertion Chimera


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2018
    Illegal XhoI site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
    Illegal AgeI site found at 938
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.


Source

The cohesin portion was PCR amplified from a plasmid provided by Dr. Steven Smith of the Queen's University Department of Biomedical and Molecular Sciences. The amplified part was used for PCR overlap extension into the flagellin variable domain.

References